精品蜜臀久久久久99网站,亚洲精品国产精华液,精品人妻无码专区在线视频,国产在线观看无码免费视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>大鼠正常細(xì)胞>CRL-1444A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

簡(jiǎn)要描述:CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2497

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1444™  Price:$275.00
Designations:A7r5

Depositors:W Carlisle

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:fibroblast

Source:Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle


Cellular Products:myokinase; creatine phosphokinase (muscle isoenzyme); myosin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1444

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Age:embryo

Comments:The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.

Muscle type CPK is synthesized after cell division has ceased.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

CRL-1444




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
女人与zzzooooxxxx| 无码粉嫩虎白一线天在线观看| 特黄特色大片免费播放器下载| 浪货趴办公桌~h揉秘书| 成人欧美一区二区三区在线| 中文字幕aⅴ人妻一区二区| 国产麻豆剧传媒精品国产av| 免费观看mv大片的直播软件| 成人精品一区二区三区电影| 玉米地被老头添的好爽| 国产又猛又黄又爽| 国产人妻久久精品二区三区特黄| 吉吉影音av| 真人性囗交69视频| 无码粉嫩虎白一线天在线观看| 国语自产少妇精品视频| 男男互攻互受h啪肉np文| 男女多p混交群体交乱| 影音先锋资源站| 亚洲精品女同中文字幕| 真人性囗交69视频| 小娟翁止熄痒禁伦短文合集俏佳人| 肥老熟妇伦子伦456视频| 无码少妇一区二区| 国产精品久久久久一区二区三区 | 韩国三级丰满少妇高潮| 亚洲精品久久久久一区二区三区| 性xxxxfreexxxxxvideo| 男人撕开奶罩揉吮奶头gif| 久久久久久久久久久国产| 日韩a片无码毛片免费看小说| 国产午夜三级一区二区三| 中文字幕人妻无码一区二区三区| 99riav国产精品视频| 在线天堂中文在线资源网| 小妖精腰细奶大h侍卫| 欧美精品国产综合久久| 亚洲国产成人精品无码区在线网站 | 黄网站色视频免费观看| 意大利电影巜丰满的欲妇| 公主从小h后必须夹玉势sm|