精品蜜臀久久久久99网站,亚洲精品国产精华液,精品人妻无码专区在线视频,国产在线观看无码免费视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞

HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞

簡(jiǎn)要描述:HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞
HTB-119 NCI-H69 H69人肺小細(xì)胞肺癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2947

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

ATCC® N
AF Gazdar
Bios
umber:
HTB-119™Price:
Designations:NCI-H69 [H69]

Depositors:


1
Shipped:frozen
Medium & Serum:See Propagation
afety Level:



Growth Properties:suspension, multicell aggregates

Organism:Homo sapiens

Morphology:floating aggregates


Source:Organ: lung
Disease: carcinoma; small cell lung cancer


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:insulin-like growth factor II (IGF II)

Tumorigenic:Yes  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

Oncogene:myc +; myb +; fes +; fms +; raf +; ras +

DNA Profile (STR):CSF1PO: 10, 12

D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

THO1: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY



Cytogenetic Analysis:modal number = 76 to 78; range = 40 to 87

This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.



Isoenzymes:AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 1



Age:55 years adult  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞

Gender:male

Ethnicity:Caucasian

Comments:This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.

The cells grow in aggregates, thus cell counts are not accurate.

The cells stain positively for cytokeratins.

The line can be adapted to grow in shaker flask or spinner flask systems.

The N-myc gene is amplified, and there is expression of the mRNA and protein.

C-myc mRNA, but not protein, is expressed at a low level.

There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended  HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞
Medium Renewal: 2 times weekly


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-11351

purified DNA:ATCC HTB-119D



References:

1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

22465: Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

22869: Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 8386707

23036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

23037: . . Cancer Res. 40: 4556-4563, 1980.

23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

23065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

24389: . . Lung Cancer 4: 155-161, 1988.

32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

HTB-119 NCI-H69 [H69]人肺小細(xì)胞肺癌細(xì)胞




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
caoporn免费视频在线| 国产麻豆剧传媒精品国产av| ass年轻少妇浓毛pics| 纯肉大根巨无霸纯黑胡椒火腿肠| 国产精品后入内射日本在线观看| 猛烈撞击灌满白浊花液h| 中文字幕一区二区人妻| 亚洲小说春色综合另类| 亚洲色大成网站www| 大又大粗又爽又黄少妇毛片| 几个东北熟妇的性经历| 中文字幕一区二区人妻| 天天爽夜夜爽夜夜爽精品视频| 滋润岳的性饥渴| 欧美变态另类牲交| 中国猛少妇色xxxxx| 亚洲午夜福利在线观看| 在线韩漫画大全免费观看| 男男肠道灌水失禁play| 国产精品成人一区二区三区| 老板办公室乳摸gif动态图| 最美情侣韩国片免费观看| 国产综合精品| 伦人伦xxxx国语对白| 色欲aⅴ亚洲情无码av| 精品无码久久久久久久久| 欧美一区二区三区| 亚洲午夜福利在线观看| 国产精品成人一区二区三区 | 亚洲精品成人a在线观看| 丰满白嫩大屁股ass| 国产麻豆剧传媒精品国产av| 我和子的与子乱视频| 国产绳艺sm调教室论坛| 少妇av射精精品蜜桃专区| 无码国产伦一区二区三区视频| 国产精品186在线观看在线播放| 成人区人妻精品一区二区不卡视频| 把插八插露脸对白内射| 性a欧美片| 玩弄chinese丰满人妻videos|